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The first chimeric mouse was produced in 1970 There are several methodologies employed in producing transgenic animals.Microinjection method used frequently but having some drawbacks like low efficiency, variable expression patterns.
The method allows an early integration of the transgene into the host DNA, which is important to ensure that transgenic DNA is apparent in all cells of the host.
Here, first isolate the piece or pieces of DNA as per requirement and clone it into a vector such as a plasmid then harvest newly fertilized eggs before the pronuclei fuse.
A pronucleus is the nucleus of the sperm cell (male) or egg cell (female) before they join to become the fertilized zygote.
Next the piece of DNA that has been isolated is placed in a syringe and injected into the pronucleus of the sperm cell.
This could happen if the gene inserts itself in an area of DNA that is not normally expressed, so a sample of the pups tissue from the tail will be taken and DNA examined.
Transgenic Animals Research Papers Essay On Chemistry In Our Daily Life
There was generation of three healthy identical female offspring.
Transgenic animals produced with the purpose of producing better and good quality breed, increased in milk yield, as well as to produce organs to meet the demand for organ transplantation.
Genetically modified animals are proving ever more vital in the development of new treatments and cures for many serious diseases.
Genotypic analyses confirmed that all cloned offspring were derived from the donor cell line.
Analysis of the milk of one of the transgenic cloned animals showed high-level production of human antithrombin III.