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In addition, each subject only consumed either the high- or low-cholesterol diet and a cross-over study in which each subject consumed both diets for 12 weeks would have improved the validity of the data.In a future study, it is recommended that more subjects of both sexes be chosen; that meals of known cholesterol content are prepared for the subjects and that their compliance with the dietary regime be monitored effectively; and that each subject acts as his/her own control by consuming both diets in a randomised, cross-over sequence.Similarly, over the same period, consumption of a low cholesterol diet did not significantly reduce plasma cholesterol concentration.
Use the questions in the Content section and the stages mentioned here to help you to develop your argument in a logical way.
Remember that not all of the questions or the stages will be relevant to every experiment.
Whilst your results may not be enough to reject the null hypothesis, they may show a trend that later researchers may wish to explore, perhaps by refining the experiment.
For this purpose, you should criticize the experiment, and be honest about whether your design was good enough.
Some individuals indicated that they had not complied with the dietary advice and were omitted from the final analysis but it was impossible to determine how accurate the exact dietary intake of each subject or even whether they had consumed high- or low-cholesterol foods.
Certainly the precise consumption of cholesterol was not monitored and could have varied from week to week.That is the main point of your discussion section, but the process is usually a lot more complex than that.It is rarely clear-cut, and you will need to interpret your findings.Consumption of a high cholesterol diet did not significantly increase blood cholesterol concentration over a 12-week period (Figure 1) Similarly, over the same period, consumption of a low cholesterol diet did not significantly reduce plasma cholesterol concentration.However after 12 weeks the plasma cholesterol concentration of the high-cholesterol diet group was 50% greater than that of the low-cholesterol group (PThe greatest problem with this study, however, was compliance.One guideline for staging the discussion is shown here based on extracts from the discussion in one of the cholesterol reports.Although this is a good guideline, remember it is only a guideline and you need to adapt it to each experiment you carry out.If you left few interesting leads and open questions in the results section, the discussion is simply a matter of building upon those and expanding them.In an ideal world, you could simply reject your null or alternative hypotheses according to the significance levels found by the statistics.If not, suggest any modifications and improvements that could be made to the design.Maybe the reason that you did not find a significant correlation is because your sampling was not random, or you did not use sensitive enough equipment.